FEI Argos


ARGOS (Automated Recognition of Geometries, Objects, and Segmentations) is a software tool developed by FEI comany that allows automated localization of objects in noisy threedimensional data sets like cryo electron tomograms. This is done using a method called template matching which is based on cross correlation between the tomogram and a model template of the structure of interest.

The correlation map between the data set and the template indicates exact position and orientation of this structure and allows fast segmentation of widespread and characteristically shaped structures like membranes (image) or microtubuli etc. This method can also be used for automated recognition and boxing of similar particles like protein complexes that can be averaged in order to obtain high resolution maps.

The image shows the process of automated segmentation of three spherical liposomes that contain antibody labeled membrane proteins on their surface. By choosing the right combination of templates, the software can disdinguish between the membrane and the connected protein which makes subjective and labor intensive by-hand-segmentation needless.

Our group is involved in the development of ARGOS.

FEI Argos